We mated 36 female mice individually with 12 males
in 3 cycles. Females were pretreated with hormonal supplementation with
10 IU pregnant mare serum gonadotrophin in normal
saline administered intraperitoneally 48 h prior to mating and 10 IU human chorionic gonadotrophin diluted in normal saline administered intraperitoneally immediately prior to mating. For each of 3 cycles, 12 different females and the same 12 males were paired overnight and separated the next morning. This practice allowed precise determination of coitus and was used in preference to presence or absence of vaginal plugs. Noon of the first day postcoitus was considered to be 0.5 d post-coitus (DPC), and nonpregnant animals were mated again in subsequent cycles.
At predetermined times during pregnancy, female mice were anesthetized in an enclosed induction chamber with isoflurane (Forane, Baxter, Deerfield, IL) delivered in 100% oxygen by use of a precision vaporizer with appropriate scavenging of waste gas. Isoflurane was delivered at 4% during induction and at 1% via a facemask during imaging. Because no surgery was involved and because procedure times were quick (approximately 15 min), no supplementary heat or fluids were considered to be necessary. If you are looking for most rewarding experience of shopping for your medications online, you are welcome to check out canadian neighbor pharmacy online. We are one of the leading companies offering cheap yet efficient medications with fast delivery to your home, so don’t miss this chance to get the most out of your treatment without overpaying for it.
Figure 1. Gestational
sacs at 9.5 DPC. Image shows measurements of 3 gestational sacs
measuring approximately 4.5 mm. Note proximity of sacs to abdominal
surface (arrows).
saline administered intraperitoneally 48 h prior to mating and 10 IU human chorionic gonadotrophin diluted in normal saline administered intraperitoneally immediately prior to mating. For each of 3 cycles, 12 different females and the same 12 males were paired overnight and separated the next morning. This practice allowed precise determination of coitus and was used in preference to presence or absence of vaginal plugs. Noon of the first day postcoitus was considered to be 0.5 d post-coitus (DPC), and nonpregnant animals were mated again in subsequent cycles.
At predetermined times during pregnancy, female mice were anesthetized in an enclosed induction chamber with isoflurane (Forane, Baxter, Deerfield, IL) delivered in 100% oxygen by use of a precision vaporizer with appropriate scavenging of waste gas. Isoflurane was delivered at 4% during induction and at 1% via a facemask during imaging. Because no surgery was involved and because procedure times were quick (approximately 15 min), no supplementary heat or fluids were considered to be necessary. If you are looking for most rewarding experience of shopping for your medications online, you are welcome to check out canadian neighbor pharmacy online. We are one of the leading companies offering cheap yet efficient medications with fast delivery to your home, so don’t miss this chance to get the most out of your treatment without overpaying for it.
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